Research & Creative Works
"Behavioral Interventions to Improve the Performance of Collegiate Athletes."
Epstein-Barr Virus (EBV) is the cause of mononucleosis commonly known as "mono" or "the kissing disease". In certain patients it is also the cause of cancer. Latent Membrane Protein 1 (LMP1) is a viral gene that alters cells to a cancerous state. The Geiser lab is determining the function of this viral gene in EBV replication in epithelial cells, the cells that line the oral and nasal cavity. Our previous studies have shown that a 2D cell culture is insufficient to investigate this role, so we established a 3D cell culture system (organotypic raft culture) that models the differentiation of epithelial cells into oral tissue.
The Geiser lab uses raft cultures with cells harboring wild-type or LMP1-deleted EBV viral genomes to investigate the role of LMP1 during EBV replication. We will utilize molecular, biochemical, and genetic approaches to look at the function of LMP1 in viral replication, cell proliferation and differentiation, and viral-cellular signaling pathways. Based on these studies we will develop a model for the role of LMP1 during EBV replication in differentiating epithelial cells and its resulting pathogenesis.
Dr. Geiser is an Undergraduate Research Fellows (UGF) Program Mentor of the South Dakota Biomedical Research Infrastructure Network (SD BRIN). Undergraduate students are encouraged to learn more about the biomedical research opportunities available through SD BRIN program at http://orgs.usd.edu/brin/undergrad.html and by contacting Dr. Geiser at firstname.lastname@example.org or 605-668-1606
Publications & Conference Papers
"Investigation of potential drug targets for Methicillin-Resistant Staphylococcus aureus infections" - The goal of this project is to develop novel antibacterial agents for methicillin/multidrug-resistant Staphylococcus aureus (MRSA) that are less prone to bacterial resistance. The hypothesis is that a class of central metabolic enzymes such as MRSA acetate kinase(ACK), MRSA fructose bisphosphate aldolase (FBPA), MRSA phosphotransacetylase (PTA), Mannitol-1-phosphate 5-dehydrogenase (M1P5D) and MRSA HPr kinase/phosphorylase(HprK/P), which are in silico essential to bacterial growth and absent in humans, are promising drug targets
"Investigation of the cellular and molecular mechanism of the pharmacological action of Bai-Lian-San-Jie-Tang (BLSJT) on endometriosis" - The goal of the second project is to investigate how BLSJT treat endometriosis at the cellular and molecular level. Once the mechanism is elucidated and effective fraction(s) is /are refined, the project becomes more promising in that it sheds light on a new class of drugs treating endometriosis, following the footprint of arteannuinm, an alternative of Quinine in malaria treatment.
McKracken Chronicles Poster