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Geiser Lab


Vicki Geiser, Ph.D., Assistant Professor of Biology, Mt. Marty College

  • Postdoctoral Fellow, Microbiology & Molecular Genetics, Brigham & Women's Hospital and Harvard Medical School, Boston, MA
  • Ph.D., Biological Sciences, University of Nebraska, Lincoln, NE
  • M.S., Biological Sciences, University of Nebraska, Lincoln, NE
  • B.S., Biological Sciences, University of Nebraska, Lincoln, NE

The Geiser Lab

Epstein-Barr Virus (EBV) is the cause of mononucleosis commonly known as "mono" or "the kissing disease". In certain patients it is also the cause of cancer. Latent Membrane Protein 1 (LMP1) is a viral gene that alters cells to a cancerous state.  The Geiser lab is determining the function of this viral gene in EBV replication in epithelial cells, the cells that line the oral and nasal cavity.  Our previous studies have shown that a 2D cell culture is insufficient to investigate this role, so we established a 3D cell culture system (organotypic raft culture) that models the differentiation of epithelial cells into oral tissue. 

The Geiser lab uses raft cultures with cells harboring wild-type or LMP1-deleted EBV viral genomes to investigate the role of LMP1 during EBV replication.  We will utilize molecular, biochemical, and genetic approaches to look at the function of LMP1 in viral replication, cell proliferation and differentiation, and viral-cellular signaling pathways.  Based on these studies we will develop a model for the role of LMP1 during EBV replication in differentiating epithelial cells and its resulting pathogenesis.

Research opportunities in the Geiser Lab

Dr. Geiser is an Undergraduate Research Fellows (UGF) Program Mentor of the South Dakota Biomedical Research Infrastructure Network (SD BRIN).  Undergraduate students are encouraged to learn more about the biomedical research opportunities available through SD BRIN program at http://orgs.usd.edu/brin/undergrad.html and by contacting Dr. Geiser at vicki.geiser@mtmc.edu or 605-668-1606.

Selected Peer-reviewed Publications

Research Papers
  1. Inman, M., Zhang, Y., Geiser, V., and Jones, C.  2001.  The zinc finger in the bICPO protein encoded by bovine herpesvirus 1 mediates toxicity and activates productive infection.  Journal of General Virology, 82(Pt 3):483-92, PMID: 11172088.
  2. Geiser, V., Inman, M., Zhang, Y., and Jones, C. 2002.  The latency related gene of bovine herpesvirus–1 can inhibit the ability of bICP0 to activate productive infection.  Journal of General Virology, 83(Pt 12):2965-2971, PMID: 12466472.
  3. Geiser, V., and Jones, C.  2003.  Stimulation of bovine herpesvirus-1 productive infection by adenovirus E1A gene and a cell cycle regulatory gene, E2F-4.  Journal of General Virology,84(Pt 4):929-38., PMID: 12655094
  4. Geiser, V., Zhang, Y., and Jones, C. 2005.  Analysis of a bovine herpesvirus 1 recombinant virus that does not express the bICP0 protein.  Journal of General Virology¸ 86(Pt 7):1987-96, PMID: 15958678.
  5. Geiser, V., and Jones, C.  2005.  Localization of sequences within the latency-related gene of bovine herpesvirus 1 that inhibit mammalian cell growth.  Journal of Neurovirology, 11(6):563-70, PMID: 16338750.
  6.  Zhang, Y., Jiang, Y., Geiser, V., Zhou, J., and Jones, C.  2006.  The bovine herpesvirus 1 (BHV-1) immediate early protein (bICP0) interacts with the histone acetyltransferase p300, and p300 stimulates productive infection and gC promoter activity.  Journal of General Virology, 87 (Pt 7): 1843-51, PMID: 16760386.
  7. Meyer, F., Perez S., Geiser, V., Sintek, M., Inman, M., and Jones, C.  2007. A protein encoded by the bovine herpes virus 1 (BHV-1) latency related gene interacts with specific cellular regulatory proteins, including the CCAAT enhancer binding protein alpha (C/EBP-{alpha}).  Journal of Virology, 81(1):59-67, PMID: 16987965.
  8. Geiser V., Rose S., and Jones C. 2008. Bovine herpesvirus type 1 induces cell death by a cell-type-dependent fashion. Microbial Pathogenesis, 44 (2008) 459-466, PMID: 18222625.

Reviews
  1. Jones, C., Geiser, V., Henderson, G., Jiang, Y., Meyer, F., Perez, S., Zhang., Y. 2006.  Functional analysis of bovine herpesvirus 1 (BHV-1) genes expressed during latency.  Veterinary Microbiology, 113:199-210, PMID: 16352404.


Abstracts (last five years)

  1. Geiser, V., and Jones, C.  2006.  Analysis of the cytotoxicity of a bovine herpesvirus 1 recombinant virus that does not express the bICP0 protein.  International Herpesvirus Workshop.
  2. Geiser, V., Cahir-McFarland, E., and Kieff, E. 2007. The role of Latent Membrane Protein 1 during Epstein-Barr Virus replication in epithelial cells. American Association for Cancer Research (AACR) Pathobiology of Cancer Workshop.
  3. Geiser, V., Cahir-McFarland, E., and Kieff, E. 2008. LMP1 is not required for lytic EBV replication in epithelial cells. 13th Biennial Conference of the International Association for Research on EBV and Associated Disease.
  4.  Geiser, V., and Kieff, E. 2009. Development of a reporter cell line to quantify Epstein-Barr Virus. International Herpesvirus Workshop.
  5. Geiser, V., Cahir-McFarland, E., and Kieff, E. 2009. Latent Membrane Protein 1 is not required for lytic Epstein-Barr Virus replication in epithelial cells. American Cancer Society Postdoctoral Fellows Symposium.